Ex vivo compound prioritization for gastroenteropancreatic NETs

Andrea Califano, PhD

Year: 2015
Institution: Columbia University in the City of New York
Country: United States
State: NY
Award Type: NETRF GRANTS 2005-2017
NET Type: Multiple
Science Type: Translational

General Description

Utilizing a newly established cell line (there are only 2 in the world), we will determine proteins that mediate tumor growth by standard molecular biologic techniques. Once these targets are identified, we will use combinations of targeted therapies to inhibit growth in a novel model of liver metastasis in mice. We will also establish new cell lines, as we have a large clinical practice treating patients with carcinoid tumors. Identification of molecular targets will allow for the rapid development of new therapies to be tested in subsequent years in clinical trials by Dr. James Yao and colleagues.


Attempted to create neuroendocrine cell lines. Funding from NETRF supported Lee Ellis’s work that provided a new cell line for study by numerous labs around the world. Initial studies identified new targets for therapy that can be tested in clinical trials. NETRF funding supported the first study reporting on cancer stem cells in carcinoid tumors. Cancer stem cells are thought to be the originating cells in cancers that mediate resistance to therapies and metastasis.


Van Buren G, Rashid A, Yang AD, Abdalla EK, Gray MJ, Liu W, Somcio R, Fan F, Camp ER, Yao JC, Ellis LM. The development and characterization of a human midgut carcinoid cell line. Clin Cancer Res. 2007 13(16): 4704-4712.


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