Establishment and genome characterization of PNET mouse models

Year: 2020
Institution: University Health Network
Country: Canada
Award Type: Pilot
NET Type: Pancreas
Science Type: Basic

General Description

Researchers will generate mouse models of carcinoid using conditional genetics. These mouse lines will permit investigation of tumor pathogenesis and create tumor-derived cell lines. Collectively these novel reagents will facilitate genomic, physiologic, and pharmacologic studies of carcinoid.

Results

Dr. Kim explored the use of mice to reconstruct tumors resembling carcinoids with a goal to produce a carcinoid cell line. His strategy was based on prior successful creation of tumors and tumor cell lines from a variety of mouse hormonal cells in the intestines and pancreas. He used a genetic approach based on the idea that carcinoid likely originates from specific intestinal cells producing the TPH gene. Thus far, Dr. Kim has not produced useful tumors or tumor cell lines resembling carcinoid but is continuing this approach, even after cessation of funding from  NETRF.